Figure 7 from Nanobody-Engineered CLL-1 CAR T Cells: Optimizing Tumor-Specific Cytotoxicity and Minimizing Off-Tumor Toxicity

CD33 CAR T cells, but not CLL-1 CAR T cells, exhibit myelotoxicity in vitro. CLL-1 and CD33 CAR T cells, as well as untransduced (Unt.) T cells, were cocultured with donor-matched BM-derived CD34+ hematopoietic progenitor cells at an E:T ratio of 10:1 under hematopoiesis-inducing conditions for 14 days. A, Representative image of BFU-E formation after coculture of CD34+ cells with CAR T cells. B, Representative image of CFU-GM formation. C, Quantification of BFU-E formation. D, Quantification of CFU-GM formation. E, Quantification of total CFU formation. Data represent mean values of triplicates ± standard deviation (SD). **, P < 0.0025; ns, not significant.