Detection and isolation of a microbial cell using a single-cell dispenser

This short video show the detection and isolation of a microbial cell using a single-cell dispenser.It is a sequence of:Brightfield detection - set size selectedFluorescence detection - fluorescence threshold selectedOverlap of both selections - size and fluorescence thresholds selectedCell is platedThe fluorescence of the cell was achieve by fluorescence in situ hybridization transcript-annealing molecular beacons (FISH-TAMP).