Abstract WP289: Plasma Proteomics Nominate Biomarker Candidates Of Atrial Fibrillation In Stroke Patients

Introduction: Atrial fibrillation (AFib) is a major risk factor for ischemic stroke (IS). Early AFib diagnosis is critical to optimize secondary prevention and reduce recurrent stroke risk. Objectives: We performed a proteomics study to identify plasma biomarkers associated with a history of AFib in patients with IS or TIA. Methods: We used clinical and proteomics data of stroke patients aged ≥18 years lodged within prospective plasma repositories at Grady Memorial Hospital (GMH) and Yale New Haven Hospital (YNHH) from 2010 to 2020. Plasma was collected at emergency room presentation before any intervention at GMH and within 72 hours of stroke onset at YNHH. We measured differentially abundant proteins (DAPs) between patients with or without AFib history using aptamer-based 7K SomaScan proteomics. Batch effects between centers were corrected using the ComBat function ( sva package in R). The DAPs were identified using ±1.5-fold change and unadjusted p-value