Hesperidin Inhibits Proliferation and Metastasis of Human Breast Cancer Cells by Suppressing MMP-2/9 Activity and Reversing Epithelial-Mesenchymal Transition
Abstract
Objectives: To investigate the mechanism by which Hesperidin (Hsp) inhibits the proliferation and metastasis of the triple-negative breast cancer cell line MDA-MB-231. Methods: The effect of Hsp on cell viability was analyzed using the MTT assay. The anti-migration and anti-invasion abilities of Hesperidin were evaluated using the scratch test and Transwell migration assay. The activity of matrix metalloproteinases (MMP)-2/MMP-9 was analyzed using gelatin zymography. The expression of epithelial-mesenchymal transition (EMT)-related proteins (Vimentin, Snail, ZO-1) was detected by Western blot. Results: Low concentrations of Hsp (2-10 μmol/L) showed no significant cytotoxicity; 20-40 μmol/L significantly reduced cell survival rate (p
Fields of Study & Subjects
Fungal Biology and Applications
Protease and Inhibitor Mechanisms
Seaweed-derived Bioactive Compounds
Publication details
- Journal / source
- Cancer Informatics
- Publisher
- SAGE Publications
- Volume
- 25
- Pages
- 11769351261456238–11769351261456238
- Publication date
- 1 May 2026
- Language
- EN