Evaluation of the efficacy of ozonated olive oil over commercially available toothpastes in remineralisation of white spot lesions: An in vitro study

INTRODUCTION: During the course of orthodontic treatment, a proper hygiene protocol is advised to all patients. Maintenance of oral hygiene prevents potential damage to hard tissue along with soft tissue. Despite preventive oral hygiene measures during fixed orthodontic therapy, some patients develop white spot lesions (WSL), compromising aesthetics and posing a potential risk of developing a carious lesion. Tooth remineralisation using calcium, fluoride, phosphate and other biocompatible agents is a potential solution. AIMS: This study aimed to compare the remineralising potential of Remin Pro (hydroxyapatite), Sensodyne Pronamel (sodium fluoride) and Pur O3 Olive (ozonated olive oil) on artificially created WSLs using quantitative light-induced fluorescence (QLF) and polarised light microscopy (PLM). METHODOLOGY: An in vitro study was conducted over 1 year using 75 extracted premolars, divided into three groups (n = 25). Artificial carious lesions were created with a demineralising solution, followed by a 21-day pH cycling regime with respective agents. QLF assessed lesion volume (∆Q), fluorescence loss (∆F) and maximum fluorescence loss (∆F Max), while PLM was used to evaluate the enamel subsurface changes qualitatively. RESULTS: = 0.001). There was no statistically significant difference in treatment effect between Remin Pro and Pur O3 Olive. CONCLUSION: Remin Pro demonstrated greater remineralisation potential compared to Sensodyne Pronamel and Pur O3 Olive under in vitro conditions. There was no statistically significant difference in treatment effect between Remin Pro and Pur O3 Olive.