Effects of N–1-Mer Impurities in Antisense Oligonucleotides on the Target RNA Suppression

Nucleic Acid Therapeutics 2026 Open access

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Authors: Tomoka Akita, Elisa Tomita-Sudo, Renshin Sano, Nae Sakimoto, Shigenori Iwai, Hirokazu Nankai, Obika Satoshi, Takao Inoue, Junji Kawakami
DOI: 10.1177/21593337261419436
Cited by: 1
References: 40

Abstract

In this study, we focused on N–1 impurities in antisense oligonucleotides. We evaluated their binding affinity to the therapeutic target RNA, which had the complementary sequence to the full-length N-mer desired product (DP), and their ability to recruit ribonuclease H (RNase H) using cell-free in vitro assays. The binding affinity of each N–1-mer to the target RNA was extremely low, with binding constants

Fields of Study & Subjects

DNA and Nucleic Acid Chemistry RNA Interference and Gene Delivery Bacterial Genetics and Biotechnology

Publication details

Journal / source
Nucleic Acid Therapeutics
Publisher
SAGE Publications
Volume
36
Issue
2
Pages
109–116
Publication date
4 February 2026
Language
EN