DNA methylation-based immune cell profiling in mouse blood using MouseRS-CMD

Genome biology 2026 Open access

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Authors: Samuel R. Reynolds, Hannah G. Stolrow, Min Kyung Lee, Steven C. Pike, Fred W. Kolling, Jacqueline Y. Channon, Mielcarz Daniel W., Gary A. Ward, Steven Fiering, Jennifer Fields, Lucas A. Salas, Brock C. Christensen
DOI: 10.1186/s13059-026-04121-y
Cited by: 1
References: 36

Abstract

We present MouseRS-CMD, a murine DNA methylation-based immune cell deconvolution tool. Neutrophils, monocytes, natural killer cells, B cells, CD4 + and CD8 + T cells were purified. DNA methylation was measured with the Illumina Mouse Methylation BeadChip at > 285,000 CpG loci for reference library development and in eye and terminal bleed whole blood with flow cytometry measurements for validation. The IDOL algorithm identified an optimal reference library of 300 CpGs with RMSE < 2.7 for four cell types and < 3.5 for all cell types. MouseRS-CMD enables immune profiling in archival samples and reduces confounding from cell type heterogeneity in molecular studies.

Fields of Study & Subjects

Single-cell and spatial transcriptomics Immune cells in cancer Cell Image Analysis Techniques

Publication details

Journal / source
Genome biology
Publisher
Springer Science and Business Media LLC
Volume
27
Issue
1
Publication date
5 June 2026
Language
EN