Cancer-associated fibroblast-derived exosomal LINC00930 represses cell malignancy and glycolysis by recruiting MBNL1 and elevating PRKAA2 stability in colorectal cancer

Exosomes (EXOs) secreted by cancer-associated fibroblasts (CAFs) can induce malignant phenotypes of tumor cells. The long non-coding RNA LINC00930 is known for promoting cancer cell glycolysis and proliferation. However, its role and mechanism in colorectal cancer (CRC) are unclear. This study aimed to determine whether CAF-derived exosomal LINC00930 is involved in CRC progression. In our study, LINC00930 was significantly downregulated in CRC samples and cell lines, as well as in CAFs and CAF-derived EXOs. Functionally, CAF-derived exosomal LINC00930 inhibited CRC cell proliferation, migration, invasion, and glycolysis in vitro and impaired tumor growth in patient-derived xenograft models. LINC00930 improved PRKAA2 stability by recruiting MBNL1. Furthermore, PRKAA2 silencing partly reversed CAF-derived exosomal LINC00930-mediated effects on CRC cell proliferation, migration, invasion, and glycolysis. In conclusion, this study identifies LINC00930 as a tumor-suppressive lncRNA delivered by CAF-derived EXOs that inhibits CRC progression through the MBNL1-PRKAA2 axis, suggesting that LINC00930 could be a viable therapeutic target for CRC.