Sources of Error in the Densitometric Evaluation of Thin-Layer Separations with Special Regard to Nonlinear Problems
Abstract
Thin-layer chromatography (TLC) is a highly efficient method for the separation of complex solutions into their individual components. Electrophoresis serves a similar purpose, though the techniques and fields of application of the two methods differ significantly. Of the various modalities of electrophoresis, only flat-bed separation, mainly on gel substrates, is considered here. Although originally densitometry was used mainly for qualitative analysis, it is now more and more used for quantitative determinations. Smaller and smaller sample volumes are analyzed, and the demands upon accuracy are steadily increasing.
Fields of Study & Subjects
Hermeneutics and Narrative Identity
Aging
Elder Care
and Social Issues
Health
Medicine and Society
Publication details
- Journal / source
- Advances in chromatography
- Pages
- 201–220
- Publication date
- 6 January 2026
- Language
- EN
- Document type
- Book chapter