Tryptic Mapping by Reversed Phase Liquid Chromatography

Advances in chromatography 2026 Book chapter

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Authors: Michael W. Dong
DOI: 10.1201/9781003760115-2
Cited by: 0

Abstract

This chapter has several major objectives: To review the technique of tryptic mapping by reversed phase liquid chromatography (LC) including enzymatic digestion procedures To summarize the column and instrumental requirements for reproducible, high-resolution, and high-sensitivity tryptic mapping To discuss new column trends in tryptic mapping by LC To elucidate criteria for mobile phase selection and the effects of operating variables on peptide resolution

Fields of Study & Subjects

Analytical Chemistry and Chromatography Chromatography in Natural Products Protein purification and stability

Publication details

Journal / source
Advances in chromatography
Pages
21–52
Publication date
6 January 2026
Language
EN
Document type
Book chapter