Monitoring sulfate-reducing bacteria (SRB) using molecular tools during in situ immobilization of heavy metals

Environmental Biotechnology ESEB 2004 2026 Book chapter

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Authors: Joke Geets, Brigitte Borremans, K. Vanbroeckhoven, Ludo Diels, D. Van Der Lelie, J. Vangronsveld
DOI: 10.1201/9781003762645-140
Cited by: 1

Abstract

In situ bioprecipitation (ISBP) of heavy metals as metal sulfides by the activity of sulfatereducing bacteria (SRB) is a promising strategy for sustaining groundwater quality. The feasibility of the ISBP process for a Zn,Cd,Ni and Co -polluted industrial site was studied in column experiments, using lactate, molasses or HRC Ⓡ as supplemented electron donor and C -source. In addition, the effect of extra N/P was evaluated. In order to link the results of analytical analyzes to SRB-community composition and activity, the SRB population was monitored by molecular tools such as SRB-subgroup or – genus specific PCR and DGGE of the 16S rRNA- and dsr (dissimilatory sulfite reductase) gene.

Fields of Study & Subjects

Mine drainage and remediation techniques Metal Extraction and Bioleaching Minerals Flotation and Separation Techniques

Publication details

Journal / source
Environmental Biotechnology ESEB 2004
Publisher
CRC Press
Pages
569–572
Publication date
22 January 2026
Language
EN
Document type
Book chapter