Liquid and solid state NMR study of benzothiazole degradation by Rhodococcus isolates

In this paper, recent data on biodegradative pathways of benzothiazoles by various Rhodococcus isolates ( https://www.w3.org/1998/Math/MathML" display="inline"> R . erythropolis, https://www.w3.org/1998/Math/MathML" display="inline"> R . rhodochrous, https://www.w3.org/1998/Math/MathML" display="inline"> R . pyridinovorans PA) are presented. Benzothiazole derivatives belong to a large family of xenobiotics produced world-wide in chemical industry for various applications. These pollutants are quite recalcitrant and toxic, they are found in various environmental compartments, especially in water. We used in situ 1D and 2D liquid state NMR to investigate the biodegradative pathway of benzothiazole (BT), 2–hydroxybenzothiazole (OBT) and 2–aminobenzothiazole (ABT). A common step of the degradative pathway was found corresponding to the hydroxylation of benzothiazole derivatives on the aromatic ring in position 6 . In the case of https://www.w3.org/1998/Math/MathML" display="inline"> R . pyridinovorans PA a catechol 1,2–dioxygenase activity was shown using a specific inhibitor. In a more recent study, we used https://www.w3.org/1998/Math/MathML" display="inline"> 1 H solid state NMR to study the degradation of benzothiazole-2–sulfonate ( https://www.w3.org/1998/Math/MathML" display="inline"> B T S O 3 ) by Rhodococcus erythropolis in the presence of anionic clays.