Insulin receptor expression and its association with hyperinsulinemia in triple negative breast cancer

Purpose: Hyperinsulinemia and tumor insulin receptor (IR) expression have been associated with triple negative breast cancer (TNBC) progression in preclinical models. We aimed to evaluate the expression of the IR, IGF-1 receptor (IGF-1R), and associated signaling protein expression in TNBC and their correlations with demographic and metabolic parameters in a population of women with TNBC. Methods: We identified cases of TNBC from our multi-institutional, cross-sectional study of self-identified Black and White women with newly diagnosed breast cancer. Survey, anthropometric, screening behavior, laboratory, and tumor pathology reports were collected, along with formalin-fixed paraffin embedded tumor samples. We performed immunohistochemistry (IHC) analysis and quantified the expression of IR, IGF-1R, phosphorylated Erk1/2 (pErk1/2), and FOXO3a. Clinical information was correlated with IHC scoring. Results: = .003) and was associated with body mass index and fasting insulin on univariate analysis but was not significantly associated with age. On multivariate analysis, IR expression was associated with fasting insulin but not race. IGF-1R, FOXO3a, and pErk1/2 staining were not associated with any of these factors. Conclusion: Tumor IR expression was associated with higher fasting insulin, and higher fasting insulin was more prevalent among Black women. Further studies are needed to determine the importance of hyperinsulinemia and tumor IR expression in the development of TNBC.