Feasibility, reproducibility, and cold-induced energy expenditure using whole-room calorimetry in adults and children

Abstract Context To understand energy balance, whole-room indirect calorimetry (WRIC) allows for accurate measurement of energy expenditure (EE). Objectives To examine the relationship between cold-induced resting EE and brown adipose tissue (BAT) activity measured by MRI, evaluate WRICS performance and feasibility of use in children and adults. Methods The WRICS was equipped with a Promethion High-Definition Room Calorimetry system. Technical validation utilized N2 and CO2 gas infusions. Healthy adults and children (8 years and older) attended two 4-hour WRIC visits (one week apart) and one MRI visit. Resting EE at 25°C (REE 25 ) was compared between visits and to REE at 18°C (REE 18 ). Recruitment and completion rates were examined. BAT activity was assessed by MRI as the decline in supraclavicular proton density fat fraction during 18°C cold exposure. Results Gas infusion testing confirmed high accuracy (RER=0.99; 95% CI 0.991–0.996). Study completion rates were high (Adults: 20/21; Children: 18/18). REE 25 was consistent between visits (Adults: 1.59 vs 1.63 kcal/min, p=0.56; Children: 1.57 vs 1.56 kcal/min, p=0.76) with good reproducibility (ICC Adults: 0.766; Children: 0.887). Cold exposure increased REE by 0.23 kcal/min (adults) and 0.18 kcal/min (children). BAT activity correlated with REE 18 in both groups (Adults: r=0.51, p=0.03; Children: r=0.64, p=0.03). Conclusion WRICS use was feasible in adults and children. The WRICS measurement was accurate, measures of REE were reproducible and changes in EE during cold were measurable, and related to BAT activity, supporting the usefulness of this system in the assessment of EE in response to interventions in adults and children.