[Chlorhexidine and Polident Induce the Formation of Candida albicans Persistence].

Objective: ), which is the causative agent of denture stomatitis. Methods: biofilms were formed and treated with 0-40000 μg/mL chlorhexidine and Polident. Surviving colonies were counted, and dose- and time-dependent curves were plotted. The distribution of persisters was observed via vital staining, and retention was verified by isolating surviving colonies. Transcriptomic analysis was performed on strains treated with chlorhexidine for 2 and 6 hours to explore the mechanisms of persister formation. Metabolic changes in persisters were assessed using the MTT assay. Exogenous glucose was added to validate the role of denture cleansers in regulating persister metabolism. Results: < 0.05). Conclusion: shifts from the tricarboxylic acid cycle and aerobic respiration to glycolysis to maintain a low metabolic level. Exogenous glucose can activate their metabolism and decrease the number of persisters.