Kinetics of Nitrous Oxide (N2O)-reducing Activity of Bradyrhizobium ottawaense by an Automated Analysis
Abstract
The biological reduction of N2O, a potent greenhouse gas, is crucial for environmental sustainability. We developed an automated system for continuous N2O monitoring in the gas phase of a flask containing an anaerobic bradyrhizobial culture, and then examined the kinetic parameters of bacterial N2O reduction. The maximum reaction rate (Vmax) was approximately 61-fold higher for Bradyrhizobium ottawaense SG09 (1,471 nmol h–1 109 cells–1) than for B. diazoefficiens USDA110 (24 nmol h–1 109 cells–1). Our kinetics analysis confirmed that SG09 maintained higher N2O-reducing activity than USDA110 even at the atmospheric concentration of N2O (0.34 ppm).
Fields of Study & Subjects
Legume Nitrogen Fixing Symbiosis
Microbial metabolism and enzyme function
Soil Carbon and Nitrogen Dynamics
Publication details
- Journal / source
- Microbes and Environments
- Volume
- 41
- Issue
- 1
- Pages
- n/a–n/a
- Publication date
- 1 January 2026
- Language
- EN