Morphophysiological responses of black soybean to nitrogen-fixing bacterial inoculants from diverse agroecosystems

Abstract. Hibatullah FH, Yaseen U, Ahmad F, Khumairah FH, Karuniawan A, Sofyan ET, Nurbaity A, Simarmata T. 2026. Morphophysiological responses of black soybean to nitrogen-fixing bacterial inoculants from diverse agroecosystems. Biodiversitas 27 (1): d270133. https://doi.org/10.13057/biodiv/d270133. Black soybean (Glycine max) is a legume crop of high nutritional and economic value, especially in sustainable agricultural systems. Its productivity, however, is often limited by nutrient-deficient soils. We hypothesized that inoculation with Symbiotic Nitrogen-Fixing Bacteria (SNFB) sourced from diverse agroecosystems could enhance morphophysiological performance under controlled conditions. This study aimed to evaluate the morphophysiological responses of black soybean to inoculation with SNFB isolated from diverse agroecosystems. The study comprised (i) isolation and phenotypic characterization of SNFB strains and (ii) greenhouse evaluation using a randomized complete block design with six bacterial treatments (BJ-H1, BJ-H2, BJ-H3, BJ-H4, BJ-H5, and BJ-H6) and an uninoculated control, each with four replicates. Parameters measured included plant height, chlorophyll content, fresh and dry biomass, and nodulation. Analysis of Variance (ANOVA) followed by post-hoc comparisons (p