[Cloning and expression analysis of HrNRAMP1 in Hippophae rhamnoides subsp. sinensis Rousi].

. The results of tobacco protoplast transformation indicated that HrNRAMP1 was specifically localized to the plasma membrane. RNA sequencing data (RNA-seq) and real time fluorescence quantitative polymerase chain reaction (qRT-PCR) validation showed that HrNRAMP1 expression was initially upregulated and subsequently downregulated with the rise in lead ion concentration, peaking at a lead concentration of 2 000 mg/kg. This study suggests that HrNRAMP1 may play a key role in regulating lead ion transport and responding to heavy metal stress in plants. The research suggests that HrNRAMP1 may play a key role in regulating lead ion transport and responding to heavy metal stress within plants. This finding lays a foundation for further exploration into the molecular regulatory mechanisms by which sea buckthorn responds to heavy metal lead stress, as well as for genetic engineering efforts aimed at developing new sea buckthorn cultivars with reduced accumulation of heavy metal lead.