The influence of Epstein-Barr virus on the expression of acetyl-CoA carboxylase 1 in gastric cancer cells and its underlying mechanism

Objective To investigate the effect of Epstein-Barr virus (EBV) on the expression of acetyl-CoA carboxylase 1 (ACC1) in gastric cancer cells and its potential underlying mechanism. Methods Transcriptomic and proteomic analyses were performed to identify differentially expressed genes and proteins between the human gastric adenocarcinoma cell line AGS and EBV-infected AGS (AGS-EBV) cells. The relative mRNA and protein expression levels of ACC1 in the AGS and AGS-EBV cells were determined by quantitative real-time PCR (qRT-PCR) and Western blotting (WB). A stable cell model overexpressing LMP2A (AGS-LMP2A cells) was constructed by lentiviral transduction of the AGS cells, with AGS cells transduced with an empty vector (AGS-NC cells) serving as the control. The relative mRNA and protein expression levels of ACC1 in these two cell types were measured by qRT-PCR and WB, respectively. Results Transcriptomic and proteomic analyses showed that, compared with the AGS cells, the differentially expressed genes and proteins in the AGS-EBV cells were mainly enriched in the fatty acid metabolism and fatty acid synthesis pathways, with ACC1 gene and protein expression levels significantly downregulated. The qRT-PCR and WB results showed that the relative mRNA and protein expression levels of ACC1 were significantly lower in the AGS-EBV cells than in the AGS cells (t=7.426, 2.733,P