Expression of MAD2L1 and miR-28-5p in gastric cancer tissues and their clinical significance

Objective To explore the expression levels of mitotic arrest deficient 2 like 1（MAD2L1）and microRNA（miR）-28-5p in gastric cancer tissues and their relationship with the clinicopathological characteristics of gastric cancerpatients，in order to provide a reference for early diagnosis and treatment. Methods The differentially expressed genes in gastric cancer were downloaded from the Gene Expression Omnibus（GEO）database，and MAD2L1 was identified as the target gene for this study. Based on the LinkedOmics website，the miRs related to MAD2L1 were screened，and whether there was a targeting relationship between MAD2L1 and miR-28-5p was verified using Targetscan. Tumor tissues surgically resected from 94 gastric cancer patients at the Affiliated Hospital of Xuzhou Medical University were collected from May to November 2024，along with corresponding adjacent tissues obtained at least 5 cm away from the tumor margin. The expression levels of MAD2L1 and miR-28-5p were detected by immunohistochemistry and reverse transcription quantitative polymerase chain reaction（RT-qPCR），respectively. The correlation between the two was analyzed by the Spearman correlation coefficient，and their relationship with the clinicopathological characteristics of gastric cancer patients was analyzed by the chi-squared test. Results Bioinformatics analysis revealed that MAD2L1 was upregulated and miR-28-5p was downregulated in gastric cancer. MAD2L1 had a targeting relationship with miR-28-5p. The immunohistochemical results showed that the expression rate of MAD2L1 in gastric cancer tissues（56.38%）was significantly higher than that in adjacent tissues（39.36%）（χ2=5.457，P < 0.05）. The RT-qPCR results showed that the expression level of miR-28-5p in gastric cancer（0.26 ± 0.08）was significantly lower than that in adjacent tissues（1.14±0.53）（t=16.048，P