Enhancement of accuracy and precision of cell Young’s modulus measurement: FEM optimization and algebraic compensation
Abstract
This study addresses the inaccuracy of the Hertz model in measuring single-cell Young's modulus via probe-based indentation. We propose two complementary correction strategies: finite element model optimization and an algebraic compensation algorithm. Both methods significantly enhance measurement accuracy and consistency within the biologically relevant 400-1200 Pa range (typical for adherent cancer cells). The compensation algorithm achieves a relative error below 5.2%. This work establishes a robust framework for cellular mechanical characterization, paving the way for incorporating greater biological complexity in future models.
Fields of Study & Subjects
Scientific Measurement and Uncertainty Evaluation
Force Microscopy Techniques and Applications
Advanced Electron Microscopy Techniques and Applications
Publication details
- Journal / source
- Biomedical Optics Express
- Publisher
- Optica Publishing Group
- Volume
- 17
- Issue
- 3
- Pages
- 1512–1512
- Publication date
- 18 February 2026
- Language
- EN