Whole-slide mapping of tumor tissue fiber architecture via computational scattered light imaging
Abstract
Mapping peritumoral collagen fiber directionality in solid tumors may assist in determining cancer progression and support more personalized prognoses. However, existing microscopy techniques are often limited by a restricted field of view, high cost, or incompatibility with paraffin-treated tissues. Computational scattered light imaging (ComSLI) is a cost-effective whole-slide microscopy technique that reveals fiber orientations independent of sample preparation. Using glioma, colorectal, and head and neck cancer samples, we show for the first time that ComSLI maps fiber orientations in paraffin-treated tumor tissues, visualizes tumor growth pathways and desmoplastic reactions, and allows the study of collagen orientations relative to tumor boundaries.
Fields of Study & Subjects
Advanced Fluorescence Microscopy Techniques
Optical Imaging and Spectroscopy Techniques
Cellular Mechanics and Interactions
Publication details
- Journal / source
- Biomedical Optics Express
- Publisher
- Optica Publishing Group
- Volume
- 17
- Issue
- 5
- Pages
- 2675–2675
- Publication date
- 14 April 2026
- Language
- EN