Fast wide-field light sheet electro-optic FLIM

Optics Express 2026 Open access

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Authors: Victoria Knight, Nils Bode, Dara P. Dowlatshahi, Jung-Gun Kim, Mary Beth Mudgett, Soichi Wakatsuki, Adam J. Bowman, Mark A. Kasevich
DOI: 10.1364/oe.581295
Cited by: 3
References: 26

Abstract

We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet electro-optic FLIM (LS-EO-FLIM) acquisition with up to 800 μ m field of view. Volume acquisitions are demonstrated on fluorescent bead mixtures and in live Arabidopsis thaliana root samples using both genetically encoded fluorescent proteins and endogenous autofluorescence.

Fields of Study & Subjects

Photonic and Optical Devices Magneto-Optical Properties and Applications Advanced Photonic Communication Systems

Publication details

Journal / source
Optics Express
Publisher
Optica Publishing Group
Volume
34
Issue
8
Pages
15065–15065
Publication date
3 February 2026
Language
EN