Dual-color single-molecule localization microscopy with a sub-nanometer channel misalignment and sub-1% color crosstalk

Optics Express 2026 Open access

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Authors: Yulong Sun, Xingguang Chen, Xiaoyang Tang, Yingqi Sun, Bing Shi, Zhen-li Huang, Yingjun Zhang
DOI: 10.1364/oe.593140
Cited by: 1
References: 31

Abstract

Multicolor single-molecule localization microscopy (SMLM) plays a critical role in studying biomolecular interactions at the subcellular organelle scale, owing to its nanoscale resolution. However, the study of interactions between proteins only a few nanometers in size (such as globulins and insulin) requires colocalization analysis with sub-nanometer precision. Although existing super-resolution imaging techniques have achieved breakthroughs in resolution (sub-nanometer) and color crosstalk rate (

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Near-Field Optical Microscopy Force Microscopy Techniques and Applications

Publication details

Journal / source
Optics Express
Publisher
Optica Publishing Group
Volume
34
Issue
9
Pages
16033–16033
Publication date
8 April 2026
Language
EN