Super-resolution structured illumination microscopy of cellular dynamics with fluorescence lifetime contrast

Optica 2026 Open access

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Authors: Raphaël Marchand, Henning Ortkrass, Daniel Aziz, Franz Pfanner, Eman Abbas, Silvio O. Rizzoli, Wolfgang Hübner, Adam Bowman, Thomas R. Huser, Thomas Juffmann
DOI: 10.1364/optica.589837
Cited by: 1
References: 53

Abstract

Super-resolution structured illumination microscopy (SR-SIM) enables fluorescence microscopy beyond the diffraction limit at high frame rates. Compared to other super-resolution microscopy techniques, the low photon fluence used in SR-SIM makes it readily compatible with live-cell imaging. Here, we combine SR-SIM with electro-optic fluorescence lifetime imaging (EOFLIM), demonstrating super-resolved multiplexed imaging of spectrally overlapping fluorophores, enhanced contrast due to optical sectioning, and environmental sensing at a spatial resolution of 156 nm. The high photon detection throughput of EOFLIM enables a combination of lifetime precision, size of the field of view, spatial resolution, and speed, which, taken together, is unprecedented, enabling the super-resolved imaging of cellular dynamics.

Fields of Study & Subjects

Advanced Fluorescence Microscopy Techniques Digital Holography and Microscopy Optical Imaging and Spectroscopy Techniques

Publication details

Journal / source
Optica
Publisher
Optica Publishing Group
Volume
13
Issue
6
Pages
1065–1065
Publication date
20 April 2026
Language
EN