[Effects of electroacupuncture on learning memory and central inflammatory response in rats with cerebral ischemia-reperfusion].

OBJECTIVES: To observe the effects of electroacupuncture (EA) on learning memory and central inflammatory response in rats with middle cerebral artery occlusion/reperfusion (MCAO/R), and to explore its potential mechanisms. METHODS: A total of 88 SD rats were randomly divided into a blank group (14 rats), a sham operation group (14 rats), and the remaining 60 rats were used to establish the MCAO/R model using the suture-occlusion method. The successfully modeled rats were randomly divided into a model group and an EA group, with 14 rats in each group. Rats of the EA group received EA at "Shenting" (GV24) and "Baihui" (GV20) for 30 min, once daily for 14 consecutive days. After intervention, neurological function and learning memory were evaluated using the Zea-Longa score and Morris water maze test. The brain infarction volume was assessed using TTC staining. Neuronal damage was observed using Nissl staining. Immunofluorescence was used to detect the positive expression of ionized calcium-binding adapter molecule 1 (Iba1) in the hippocampal CA1 region. ELISA was used to measure the levels of pro-inflammatory cytokines interleukin (IL)-6, IL-1β, tumor necrosis factor (TNF)-α, and the blood-brain barrier-related factor S100β in serum. Western blot was used to detect the relative expression levels of Iba1, inflammatory factors, and tight junction proteins zonula occludens-1 (ZO-1), Occludin, and Claudin5 in the hippocampal tissue. RESULTS: