[Electroacupuncture improves learning-memory ability following cerebral ischemia-reperfusion in rats by regulating mitochondrial fission/fusion of hippocampal neurons].

OBJECTIVES: To observe the effect of electroacupuncture (EA) on mitochondrial fission/fusion in rats with learning-memory impairment induced by cerebral ischemia-reperfusion (CI/R), so as to explore its potential neuroprotective mechanisms against the ischemic reperfusion injury of hippocampal neurons. METHODS: Male SD rats were randomly divided into normal, sham-operation, model, and EA groups, with 12 rats in each group. The CI/R model was established by middle cerebral artery occlusion and reperfusion by using the intraluminal suture method. After successful modeling, rats in the EA group received EA (2 Hz/10 Hz, 6 V) at "Shenting"(GV24) and "Baihui" (GV20) for 30 min, once daily for 14 consecutive days. The rats' learning-memory ability was evaluated using the novel object recognition test and Morris water maze test. The cerebral infarct volume was assessed by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. Hippocampal neuronal morphology was examined by HE staining, and the mitochondrial morphology and structure of hippocampal neurons were observed using transmission electron microscopy. The adenosine triphosphate (ATP) content in the hippocampal tissue was measured using colorimetry, and mitochondrial DNA (mtDNA) copy number was detected by qPCR. The relative protein and mRNA expression levels of mitochondrial fusion proteins 1/2 (MFN1/2), optic atrophy protein 1 (OPA1), dynamin-related protein 1 (DRP1), and fission protein 1 (FIS1) in the hippocampal tissue were detected by Western blot and qPCR respectively. RESULTS: