[Effect of electroacupuncture on the A20/NF-κB/NLRP3/GSDMD signaling pathway in rats with primary dysmenorrhea].

PubMed 2026 Open access

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Authors: Xiao Xue, Xin Liu, Yu Liu, Yan Huang, Yu Jiang, Hai-Ying Li, Hao-Lin Jin, Ming-Kun Yue, XIA Fei-yan, Zheng-Quan DU, Jing-Mei Li, Qi Deng
+1 more Yanhua Tang
DOI: 10.13702/j.1000-0607.20250062
Cited by: 0

Abstract

OBJECTIVES: To observe the effect of electroacupuncture (EA) at "Guanyuan" (CV4) and "Sanyinjiao" (SP6) on the ubiquitin-editing enzyme A20 (A20)/nuclear factor-κB (NF-κB)/NOD-like receptor protein 3 (NLRP3)/Gasdermin D (GSDMD) signaling pathway in rats with primary dysmenorrhea (PDM). METHODS: day, writhing behavior of rats was assessed. Uterine pathology was assessed by HE staining. Serum contents of prostaglandin F2α (PGF2α) and tumor necrosis factor-α (TNF-α) were measured by ELISA. Protein expression of A20, phosphorylated NF-κB p65 (p-NF-κB p65), NF-κB p65, NLRP3, Caspase-1, Interleukin (IL)-1β, GSDMD, and GSDMD N-terminal domain (GSDMD-N) in uterine tissue were detected by Western blot. RESULTS:

Fields of Study & Subjects

Acupuncture Treatment Research Studies Inflammasome and immune disorders Pain Mechanisms and Treatments

Publication details

Journal / source
PubMed
Volume
51
Issue
2
Pages
161–168
Publication date
25 February 2026
Language
ZH