Cytotoxic Activity and Apoptocic Induction of Dicloromethane Fraction Nauclea subdita on WiDr Colon Cancer Cell
Abstract
Colon cancer is one of the most deadly cancers in the world. Conventional treatments such as surgery, radiotherapy and chemotherapy are often limited, especially in metastatic cancer. Therefore, the development of alternative therapies, such as chemoprevention, becomes very important. This study aimed to test the anticancer potential of bengkal (Nauclea subdita) stem bark against WiDr colon cancer cells through cytotoxic, cell cycle, and apoptocic assays. The stem bark of bengkal was extracted using n-hexane and ethanol, then fractionated using dichloromethane (DCM). The results of the cytotoxic test using the MTT method showed that the DCM pH 3 fraction had the highest cytotoxic potential with an IC50 value of 112.81 ± 22.55 µg/mL against WiDr cells. However, the Selectivity Index (IS) value of the DCM pH 3 fraction showed low selectivity (
Fields of Study & Subjects
Biological Stains and Phytochemicals
Seaweed-derived Bioactive Compounds
Natural Products and Applications
Publication details
- Journal / source
- Biology Medicine & Natural Product Chemistry
- Publisher
- Al-Jamiah Research Centre
- Volume
- 15
- Issue
- 1
- Pages
- 285–292
- Publication date
- 21 April 2026
- Language
- EN