การชกนำแคลลสและยอดของมนเทศญปนสมวงในสภาพปลอดเชอ

Recently, interest in Japanese purple sweet potato (Ipomoea batatas) production has increased dramatically due to their flavor and nutritional content with high vitamin, anthocyanin, and sugar content. However, major production constraints are the quality and cost of planting material. Therefore, the present study used micropropagation techniques to determine the optimal medium formulation for callus induction and shoot multiplication of Japanese purple sweet potato. The experiment design was used Completely randomized design (CRD). Callus induction of leaf discs and tuber explants were cultured on MS medium containing 1.0 and 2.0 mg/L BA in combination with 0.1 1.5 or 1.0 mg/L NAA, and MS without plant growth regulators was used as a control. The explants were cultured for 5 weeks. For shoot induction experiment, explant derived from nodes were cultured on MS medium containing 0 0.25 0.5 1.0 1.5 and 2.0 mg/L BA for 5 weeks. The results showed that 100% callus induction occurred in tuber explants when cultured on MS medium containing1.0 mg/L BA and 1.0 mg/L NAA, with the highest callus diameter of 1.51 cm. For leaf discs cultured on MS medium containing 2.0 mg/L BA and 0.1 mg/L NAA, 100% callus induction with callus diameters of 1.33 cm was shown. The highest shoot length (2.66 cm) was observed in nodal explants cultured on growth regulator-free MS medium at 5 weeks and 80 % shoot induction was observed. These results provide valuable insights for developing efficient micropropagation protocols for Japanese purple sweet potato in Thailand, contributing to a more sustainable and high-quality production system.