Mechanism of lncRNA PLACT1 in regulating the proliferation of pancreatic adenocarcinoma cells through the KLF2/KIAA1522 axis.

BACKGROUND: Pancreatic adenocarcinoma (PAAD) is among the most common cancers worldwide. This study aims to investigate the role of long noncoding RNA pancreatic cancer-associated transcript 1 (LncRNA PLACT1) in PAAD cell proliferation. METHODS: . RESULTS: PLACT1 and KIAA1522 were highly expressed, and KLF2 was poorly expressed in PAAD cells. Silencing PLACT1 decreased cell proliferation, the number of cell clones, and EdU-positive cells. Mechanistically, PLACT1 inhibited KLF2 expression by recruiting EHMT2 to induce H3K9me2 in the KLF2 promoter region, resulting in reduced KLF2 enrichment at the KIAA1522 promoter and increased KIAA1522 expression. KLF2 downregulation or KIAA1522 overexpression alleviated the inhibitory effect of PLACT1 silencing on PAAD cell proliferation. PLACT1 silencing prevented PAAD tumorigenesis by regulating the KLF2/KIAA1522 pathway. CONCLUSION: PLACT1 silencing inhibited PAAD by inhibiting KLF2 and promoting KIAA1522 expression, suggesting the therapeutic effect of PLACT1 silencing on PAAD.