Effect of ART1 on the efficacy of oxaliplatin in colorectal cancer under high-cholesterol conditions.

BACKGROUND: . METHODS: Immunohistochemistry of CRC tissue revealed that, compared with that of normal blood lipids, ART1 expression in CRC tissue from patients with hypercholesterolaemia was higher. Based on CCK8 and EdU assays, we found that ART1 knockdown reduced the proliferation ability of CRC cells and decreased the volume of subcutaneously transplanted tumours in the high-CHO group. Finally, under high-CHO conditions, ART1 knockdown significantly reduced the protein expression levels of SPHK1, S1P, S1PR1, STAT3, and p-STAT3 in CT26 cells and transplanted tumours, as determined by western blotting. RESULTS: These findings suggest that under high-CHO conditions, inhibition of ART1 expression can promote the inhibitory effect of OXA on CT26 cell proliferation, which may be related to the influence of ART1 on STAT3 expression through SPHK1/S1P/S1PR1. CONCLUSIONS: This study is of great significance in improving the inhibitory effect of OXA on CRC cell proliferation in a high-CHO environment.