Age-dependent discordance in chemiluminescent immunoassay-electrochemiluminescence immunoassay correlation for 25-hydroxyvitamin D measurement reveals methodological considerations for assessment in early infancy
Abstract
- 2.281 (τ = 0.53, p < 0.001). Bland-Altman analysis revealed a constant negative bias of -4.89 ± 3.53 ng/mL [-5.66, -4.12] (mean ± standard deviation [95% confidence interval]) for ECLIA versus CLIA. Reclassification revealed an increase in VD deficiency (25(OH)D < 12 ng/mL) from 27.8% to 61.9%, and VD insufficiency (25(OH)D < 20 ng/mL) from 75.4% to 91.7%. The transition from CLIA to ECLIA led to lower measured 25(OH)D levels in newborns and infants and increased VD deficiency and insufficiency diagnoses. Method-specific differences should be considered when evaluating VD status during early infancy, particularly in infants under four days.
Fields of Study & Subjects
Vitamin D Research Studies
Biotin and Related Studies
Per- and polyfluoroalkyl substances research
Publication details
- Journal / source
- Endocrine Journal
- Publisher
- Japan Endocrine Society
- Volume
- 73
- Issue
- 4
- Pages
- 563–570
- Publication date
- 1 January 2026
- Language
- EN