The Israeli BAG

This Summary will only portray a very limited scope of the BAG proposal due to its size and more information will be available in the Global Summary. We plan establish a comprehensive structural narrative for the assembly and entry of viruses from the Bunyavirales family using X-ray crystallography, biophysics, biochemistry, and molecular virology. Using X-ray crystallography to obtain the 3D structure of GN and GC in different conformations and from different bunyavituses. We will pursue a structure of GN or GC bound to a neutralizing antibody to obtain new data for vaccine design. In the SAXS arena, we plan to continue the study on MT (microtubules) and expand the experiments with different nucleotide type and concentrations, (GTP, GDP, GMPCPP, and GMPPCP, GTP-gammaS). We will perform at selected points SEC-SAXS experiments in the presence of full-length tau (4RL). Our preliminary data revealed that 4RL promote MT assembly at 36C and of single tubulin rings at low temperatures.