Cryo-electron microscopy EPN/Grenoble BAG

ESRF is privileged to host two state-of-the-art microscopes. Our BAG unites the majority of local labs that utilise cryo-EM to address their diverse structural biology questions, including researchers from the EPN campus (IBS, ESRF, ILL and EMBL) and its vicinity (Cell & Plant Physiology Laboratory LPCV, Grenoble Institut des Neurosciences - GIN, Institute for Advanced Biosciences - IAB). In a nutshell, approximately 30 teams would greatly benefit from Krios time, proposing a pool of about 100 different candidate samples. The high demand for EM beamtime is partially met by two Glacios microscopes available on the campus, currently charged with the pre-screening, but also frequently yielding satisfactory structural insights. Nonetheless, the use of a Krios is indispensable for challenging samples (with heterogeneity or limited concentration requiring a large number of images, small proteins, etc.). Only such “difficult cases” will be selected for Krios time at the BAG level.