Genotype variant files for: Harnessing citizen science to contextualize adaptation mechanism discovery

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Authors: Laura E. Tibbs‐Cortes, Linqian Han, Ryan Benke, Puranjit Singh, Haiyan Huang, Jeremy B. Jewell, Tony N. Trieu, Zhou Tang, Kankshita Swaminathan, Xiaoyu Weng, Carson M. Andorf, Michelle Graham
+7 more Karen A. Sanguinet, Zhiwu Zhang, Laura Bartley, Yin Bao, Thomas Juenger, Jianming Yu, Li, Xianran
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Abstract

Summary: Genome-wide polymorphisms called from publicly-available switchgrass genotype data. These variants are used in a publication under review, Harnessing citizen science to contextualize adaptation mechanism discovery by Tibbs-Cortes et al. The source genotype data was described in Lovell et al. 2021 (https://doi.org/10.1038/s41586-020-03127-1) and is available at https://www.ncbi.nlm.nih.gov/bioproject/PRJNA622568.Genotype data collection: See Lovell et al. 2021 for full description. Briefly, rhizome plug samples from 789 diverse tetraploid switchgrass accessions were sequenced using Illumina HiSeq X10 and Illumina NovaSeq 6000 paired-end sequencing (2 × 150 bp) at HudsonAlpha Institute for Biotechnology and the Joint Genome Institute.Variant calling: The paired-end sequence reads were downloaded from the NCBI SRA. Reads were aligned to the AP13 v5 reference genome with BWA-mem and biallelic polymorphisms were called with bcftools. Polymorphisms with minor allele frequency above 0.05 and missing rate below 20% were retained.Funding acknowledgement: This research used resources provided by the SCINet project and/or the AI Center of Excellence of the USDA Agricultural Research Service, ARS project numbers 0201-88888-003-000D and 0201-88888-002-000D.

Fields of Study & Subjects

Bioenergy crop production and management Genetic Mapping and Diversity in Plants and Animals Genomics and Phylogenetic Studies

Publication details

Journal / source
Ag Data Commons
Publication date
20 January 2026
Document type
Dataset