Changes of biological age and related biomarkers in the context of a comprehensive geroprotective intervention: results of a prospective follow-up

Aim. To evaluate the PhenoAge and biomarkers’ changes in older adults during a 6-month lifestyle modification program. Objective evaluation of aging and the effectiveness of geroprotective interventions is an important task in preventive medicine. Phenotypic age (PhenoAge) is a promising tool for assessing biological age. Material and methods. The prospective study included a cohort of 100 individuals aged 35-55 years. All participants completed a comprehensive program including diet modification, physical activity, and stress management. Assessments were conducted at baseline, 3, and 6 months. Biological age was determined using the PhenoAge calculator (Levine ME, et al., 2018). For analysis, the cohort was stratified into quartiles by baseline PhenoAge. Nonparametric statistical methods with a Bonferroni correction were used. Results. Over 6 months of follow-up, a significant decrease in PhenoAge was observed, most pronounced in the groups with high baseline values (Q3, Q4). In Q4, the decrease was -6,73% (p