Polyene phosphatidylcholine repairs high glucose-induced human umbilical vein endothelial cells damage

Objective Exploring whether polyene phosphatidylcholine (PPC) has a reparative effect on high glucose-induced functional damage of human umbilical vein endothelial cells (HUVECs). Methods MTT assay was used to detect the effect of PPC on the proliferation activity of HUVECs damaged by high concentration of glucose. Cell scratch assay and Transwell migration assay were used to investigate the effect of PPC on the migration ability of high glucose damaged HUVECs. The angiogenesis experiment was conducted to investigate the effect of PPC on the ability of high concentration glucose-induced HUVECs to differentiate into blood vessels. Western blot was used to detect the effect of PPC on the expression of Bax, Bcl-2, caspase-3, and VEGFA proteins in HUVECs damaged by high concentration of glucose. Results PPC significantly restored the inhibitory effect of glucose on HUVECs proliferation and significantly promoted the recovery of high glucose-damaged HUVECs migration ability. PPC also significantly promoted the recovery of the ability of high glucose-damaged HUVECs to differentiate and form blood vessels. Western blot results showed that PPC inhibited the expression of proapoptotic proteins Bax and caspase-3 in HUVECs tissue. Conclusions PPC promotes the proliferation, migration, and vascular differentiation of high glucose-induced HUVECs by increasing VEGFA expression and inhibits apoptosis of HUVECs damaged by high concentration of glucose through Bax/Bcl-2/caspase-3 signaling pathway.