Effects of sodium selenite, zinc sulfate and chromium trichloride on the En-richment and Growth of Saccharomyces boulardii L2

Background.Selenium, zinc, and chromium are essential trace elements for maintaining human and animal health.However, their free ionic states exhibit low bioavailability and significant toxicity.Saccharomyces cerevisiae serves as an ideal carrier for trace element enrichment, which is due to its rapid proliferation and efficient biotransformation capabilities.Methods.Using Saccharomyces boulardii L2 as a model, this study systematically analyzed the effects of sodium selenite, zinc sulfate, and chromium trichloride concentration on Saccharomyces boulardii L2 growth and element enrichment through adding single Na 2 SeO 3 , ZnSO 4 and CrCl 3 and their combination.Scanning electron microscopy (SEM) was employed to observe changes in cell surface. Results.The optimal individual concentrations for sodium selenite, zinc sulfate, and chromium trichloride were 30 μg/mL, 300 μg/mL, and 300 μg/mL.The enrichment of Se, Zn and Cr per gram of dried Saccharomyces boulardii L2 cells was 1,060.59μg, 3,164.76μg and 1,504.95μg, respectively.Synergistic effects were observed in composite enrichment, with no elemental antagonism detected.Conclusion.Saccharomyces boulardii L2 efficiently enriches single sodium selenite, zinc sulfate and chromium trichloride and their combination system, which thereby provides critical process parameters and theoretical support for developing efficient and low-toxicity trace element yeast preparations.