Effects of Lactobacillus rhamnosus probiotic supplementation on gut health in rabbits

AIM: The research examined the effects of prolonged oral Lactobacillus rhamnosus intake on rabbit intestinal tissue morphology. METHODS: The study included twelve male rabbits aged 10–12 months and weighing 1.5–2.0 kg who received random assignment to two groups of six animals each. The control group received distilled water but the experimental group received L. rhamnosus orally at 0.25 mL/kg/day for 45 days. The research team performed histological and morphometric analysis on duodenal samples to measure villus height and width and crypt depth and villus area and VH/CD ratio and muscularis thickness through ImageJ software. RESULTS: The extended probiotic treatment caused villus disorganization and epithelial necrosis and villus fusion and inflammatory infiltration and submucosal edema. The quantitative analysis showed that all measured parameters including villus height and VH/CD ratio and villus area and muscularis thickness demonstrated significant decreases (p