Genotyping of variants rs2279238 LXRA and rs2695121 LXRB by qPCR v1

Jacqueline Soares Barros Bittar; Nayane Soares de Lima; Caroline Christine Pincela da Costa; Ângela Adamski da Silva Reis; Rodrigo da Silva Santos

doi:10.17504/protocols.io.14egn13xmv5d/v1

Genotyping of variants rs2279238 LXRA and rs2695121 LXRB by qPCR v1

2026 Preprint Open access

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Authors: Jacqueline Soares Barros Bittar, Nayane Soares de Lima, Caroline Christine Pincela da Costa, Ângela Adamski da Silva Reis, Rodrigo da Silva Santos

DOI: 10.17504/protocols.io.14egn13xmv5d/v1

Cited by: 0

Abstract

LXRA and LXRB encode the oxysterol-activated nuclear receptors LXR-alpha and LXR-beta, respectively, which are key regulators of lipid metabolism and neuroprotection. These receptors maintain lipid homeostasis by controlling the transcription of target genes involved in lipid metabolism, storage, transport, and cholesterol efflux - processes that are particularly relevant within the central nervous system. The protocol aims to standardize the genotyping of single nucleotide variants (SNVs) using a quantitative PCR (qPCR) approach.

Fields of Study & Subjects

Cholesterol and Lipid Metabolism Pharmacogenetics and Drug Metabolism Peroxisome Proliferator-Activated Receptors

Publication details

Publication date: 12 January 2026
Document type: Preprint