Large-Scale Fluorescence-Based Mammalian Cell Reporter Assay v1
Abstract
Where a scalable fluorescence-based reporter assay in HEK293 cells (using the Bxb1 landing-pad system) has been validated, this protocol describes how to perform a high-throughput screen and subsequently generate a variant effect map using TileSeq.
Fields of Study & Subjects
CRISPR and Genetic Engineering
Gene Regulatory Network Analysis
Virus-based gene therapy research
Publication details
- Publication date
- 26 January 2026
- Document type
- Preprint