Immunostaining v1

This protocol describes an immunofluorescence staining procedure with hydrogen peroxide quenching to reduce endogenous peroxidase activity, optimized for detection of TH and Myc/Cherry signals. Tissue sections are permeabilized with PBS-T, quenched with 3% H₂O₂ in the dark, and blocked with BSA before overnight incubation with primary antibodies against tyrosine hydroxylase (TH) and Myc. Fluorescent secondary antibodies and NeuroTrace are then applied for visualization, with thorough PBS-T washes between steps to minimize background. The protocol supports robust, specific labeling with reduced autofluorescence and background.