Collection and storage of myometrium, decidua, fetal membranes, and placenta (RNAlater-treated and snap frozen) tissue samples with cervicovaginal fluid sampling v1

This protocol was developed as part of Dr Brendan Browne's 'Origins of Labour' PhD project in collaboration with the Borne Uterine Mapping Project (BUMP) feasibility study; specifically for the goal of storing human tissues of the uterine environment (obtained at Caesarean section) to later analyse using RNA-seq and SWATH-MS. Samples of eight different tissue types were collected; namely myometrium (upper segment basalis region, upper segment parietalis region, and lower segment), decidua (basalis and parietalis region), amnion, chorion, and placenta. Cervicovaginal swab samples were also collected (and cervical length measurements were acquired using a transvaginal ultrasound (TVUS) scanner).