Wastewater Concentration Using the Ceres Nanotrap A Particles, Total Nucleic Acid Extraction, and PCR Inhibitor Removal for recovery of viral pathogens v1

This standard operating procedure (SOP) sets out the workflow for processing wastewater samples for with the goal of recovering and detecting viral pathogens. The steps include virus concentration using the Ceres Nanoparticles A method and total nucleic acid extraction using MagMAX Wastewater Ultra Nucleic Acid Isolation Kit The raw wastewater/sewage samples are concentrated using the Ceres Nanoparticles A method, adapted to 40 mL starting volume from the Ceres Nanotrap A 35 mL Manual Protocol with MagMAX Wastewater Ultra Nucleic Acid Isolation Kit. The samples are mixed with Nanotrap A particles and Enhancement Reagent 3, incubated on a shaker, and then pelleted on a magnetic rack. The supernatant is removed, and the beads are resuspended in Nanotrap Buffer 2, and pelleted once more. The pellet is then resuspended in lysis binding solution from the extraction kit, incubated at 56°C, and recaptured on the magnetic rack. The final lysate from the concentration step is then removed, leading into automated or manual extraction.