Programmable macromolecule delivery via engineered trogocytosis v1

Trogocytosis, the transfer of plasma membrane fragments during cell-cell contact, offers potential for macromolecular delivery. This protocol described a method to engineer donor cells with designed receptors specific to surface ligands on recipient cells, enabling cell-to-cell protein transfer through direct contact. This step-by-step protocol outlines the workflow for producing donor and recipient cells, optimized co-culture conditions and details for measurement of transfer outcomes. For complete details on the results and validation of this protocol, please refer to our work published in Nature Cell Biology.