Metabolites, Lipids, Protein extraction protocol (MPLEx) v2

2026 Preprint Open access

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Authors: Heather Olson, Ernesto Nakayasu, Wei-Jun Qian, Christopher Anderton, Gérémy Clair
DOI: 10.17504/protocols.io.36wgqd15ovk5/v2
Cited by: 1

Abstract

This protocol describes a solvent-solvent extraction method to obtain Metabolites, Lipids and Proteins from the same sample. This method, termed MPLEx, can be used on a variety of samples including biofluids (e.g., bronchoalvolar lavage fluid, plasma, blood, urine), mammalian tissues, bacteria, viruses, soil, etc.

Fields of Study & Subjects

Metabolomics and Mass Spectrometry Studies Advanced Proteomics Techniques and Applications Analytical chemistry methods development

Publication details

Publisher
Springer Science and Business Media LLC
Publication date
30 January 2026
Document type
Preprint