Phytophthora capsici CTAB DNA Extraction for High-Molecular Weight DNA v1

This protocol describes a CTAB-based DNA extraction optimized forPhytophthora capsicifrom as little as ~20 mg of mycelium, modified from protocols.io (dx.doi.org/10.17504/protocols.io.3rsgm6e), to maximize DNA integrity and purity while minimizing mechanical shearing and co-extraction of polysaccharides and phenolic contaminants. Typical yields are ~50 µL at ~300 ng/µL, with the majority of DNA >25 kb when handled without vortexing and with minimal pipetting.