GenomeTrakr WGS Protocol Collection and Workflow for MiSeq v2

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Authors: Tina Lusk Pfefer, Julie Haendiges, Maria Balkey, Candace Hope Bias, Ruth Timme
Cited by: 0

Abstract

Here we have created a collection of all the protocols used for WGS using the MiSeq, in order, from sample extraction to NCBI submission. This collection has three sections: Section 1: WGS Wet lab workflow for Illumina MiSeq Section 2: Dry lab workflow for sequence QC and NCBI submission - Direct Submission Section 3: Dry lab workflow for sequence QC and NCBI submission - PulseNet labs Version history: V5: Links to protocols updated with latest version V4: Links to protocols updated with latest version, and Step 13 - Removed protocol "Populating NCBI template for submissions using BioNumerics", and replaced with protocol "Populating metadata templates for NCBI submissions using PulseNet 2.0" V3: Links to protocols updated with latest version, Added new protocol: DNA Extraction from Inoculated Broth for WGS using the Maxwell RSC Cultured Cells DNA Kit, Added new protocol: Querying for Bacterial Pathogen Genomic Data at NCBI.

Fields of Study & Subjects

Genomics and Phylogenetic Studies Salmonella and Campylobacter epidemiology Bacterial Identification and Susceptibility Testing

Publication details

Publication date
14 January 2026
Document type
Preprint