Determination of LD50 and LD90 for Bacterial Pathogens in Galleria mellonella v1

This protocol describes a method for determining the median and 90% lethal doses (LD₅₀ and LD₉₀) of bacterial pathogens using the Galleria mellonella larvae (greater wax moth) infection model. The protocol is optimized for uropathogenic Escherichia coli (UPEC) but is readily adaptable to other bacterial species. Expected results include Kaplan-Meier survival curves for each bacterial dose and precise LD₅₀/LD₉₀ values with confidence intervals, calculated via Probit or Spearman-Kärber analysis. These metrics allow for direct comparison of pathogen strains and establish standardized infection doses for later experiments.